Gene silencing by RNA interference : technology and application

Content: Gene silencing by RNA interference and the role of small interfering RNAs --
Basics of siRNA design and chemical synthesis --
Oligonucleotide scanning arrays in the design of small interfering RNAs --
siRNA production by in vitro transcription --
Production of siRNAs with the application of deoxyribozymes --
Production of siRNA in vitro by enzymatic digestion of double-stranded RNA --
Plasmid-mediated intracellular expression of siRNAs --
Lentiviral vector-mediated delivery of si/shRNA --
Exogenous siRNA delivery: protocols for optimizing delivery to cells --
RNAi in drosophila cell cultures --
RNAi in caenorhabditis elegans --
Delivery of RNAi reagents in C. elegans by microinjection --
RNAi in drosophila embryos --
RNAi in xenopus laevis oocytes --
RNAi in xenopus embryos --
An RNAi-based genomic library for forward genetics in the African trypanosome --
RNAi in the malaria parasite plasmodium --
Silencing the expression of cysteine proteases (falcipains) in plasmodium falciparum using RNA interference approaches --
RNAi in transgenic animal models --
RNA interference in mouse models --
Virus-induced RNA silencing in plants --
RNA silencing in plants: biolistic delivery of RNAi reagents --
Agro-infiltration: a versatile tool for RNAi studies in plants.